Plasmid_Backbone

Part:BBa_K3653021:Design

Designed by: Hadas Yung, Ilana Elizarov   Group: iGEM20_Technion-Israel   (2020-10-12)


p416-FEC-mCore1 with His-tag and ACE2 enzyme


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4828
    Illegal PstI site found at 3317
    Illegal PstI site found at 3326
    Illegal PstI site found at 4260
    Illegal PstI site found at 7304
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4828
    Illegal PstI site found at 3317
    Illegal PstI site found at 3326
    Illegal PstI site found at 4260
    Illegal PstI site found at 7304
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4828
    Illegal BglII site found at 3533
    Illegal BamHI site found at 2795
    Illegal BamHI site found at 4098
    Illegal XhoI site found at 5480
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4828
    Illegal PstI site found at 3317
    Illegal PstI site found at 3326
    Illegal PstI site found at 4260
    Illegal PstI site found at 7304
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4828
    Illegal PstI site found at 3317
    Illegal PstI site found at 3326
    Illegal PstI site found at 4260
    Illegal PstI site found at 7304
    Illegal NgoMIV site found at 6023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Yeast related: High-copy-number Pmb1 ORI, URA3 (selective marker), CEN/ARS (S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence), SPG5 terminator, Lac promoter, His tag.

Bacterial related: f1 ORI, AmpR promoter, Amp (selective marker).

Modifications: ACE2 was inserted instead of yeCitrine, His-tag was added dawn stream to it.



Source

This is a modification of knowen plasmid (p416-FEC-mCore1), the original plasmid can be found in NCBI: https://www.ncbi.nlm.nih.gov/nuccore/NM_021804.1/"


References